The two views are related by a 180° rotation around the bold black line axis. Hydrogen bonds are illustrated by dashed yellow lines. Additionally, amino acid residues that form hydrogen bonds within a 3.3-Å radius of the nearest atom of the flavonoids are also shown. Amino acid residues located within a 4.0-Å radius of the nearest atom of the flavonoids are shown. A water molecule interacting with each ligand is represented with a yellow sphere. Oxygen, nitrogen, and sulfur atoms are colored red, blue, and yellow, respectively. C Amino acid residues interacting with DMG. All of the data points in duplicate assays are indicated. Each relative activity is defined as the ratio of activity compared between the respective proteins without DMG. B Inhibition of the GSH conjugation activities of wild-type AeNobo (WT, blue dots and black solid curves) and the mutated AeNobo substituting Glu-113 with Ala (E113A, red dots) using 3,4-DNADCF in the presence of DMG. A Chemical structures of DMG, also known as 4′,6,7-trihydroxyisoflavone. Desmethylglycitein (DMG) inhibits AeNobo.
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